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To verify the accuracy of the method, several artefacts that were datable by other techniques were tested; the results of the testing were in reasonable agreement with the true ages of the objects.

Over time, however, discrepancies began to appear between the known chronology for the oldest Egyptian dynasties and the radiocarbon dates of Egyptian artefacts.

Research has been ongoing since the 1960s to determine what the proportion of in the atmosphere has been over the past fifty thousand years.

The resulting data, in the form of a calibration curve, is now used to convert a given measurement of radiocarbon in a sample into an estimate of the sample's calendar age.

Animals eat the plants, and ultimately the radiocarbon is distributed throughout the biosphere.